The Gabba gang and DJ Lance Rock son are on a mission to stop a evil witch from taking over the world.
Twenty-Five years following their preschool television shows cancellation in 2015, Plex the Magic Yellow Robot, Toodee the Blue Cat-Dragon, Brobee the Little Green Monster, Foofa the White (former Pink) and Happy Flower-Bubble and Muno the Tall and Friendly Red Cyclop have been living off the finances of their reruns on TV. Their home, Gabbaland, has been destroyed by deforestation, Plex has lost his ability to magic and the Gabba gang leader, DJ Lance Rock died 2 years ago and now his son, DJ Junior Rock, spending time taking care of his father toy friends. Meanwhile, their archenemies Mother Witch, her sons, Lord Tigress and Dracula have lost power in Mount Olympus following the end of the Cold War. However, the three escape to a real-world Hollywood film studio, where they trick executive Zoe Sanderson into signing a contract giving her rights to the show, transforming the villains from preschool animated forms into live-action characters. 6 months later, in Washington D.C., CIA agent Maya Camlins and her superior, Cappy Markie, inform President Obama the 3rd that Mother Witch intends to make herself President of the United States by brainwashing television viewers using her own cable television network named "YGGTV" ("Yet Gas Goo Television"), which airs mind-numbing programming designed to zombify the public and persuade them to elect her as president. Maya is sent to a special lighthouse to bring DJ Junior and the Gabba gang into a real world by literally greenlighting a film starring the pair. Mother Witch is informed that Yo Gabba Gabba have returned and sends Lord Tigress and Dracula to destroy them. The two spies are given a weapon called the CDI ("Computer Degenerating Imagery"), which removes preschool characters from the real world by sending them to the internet. Maya and DJ Junior steals the villains truck, but are subsequently arrested by an Oklahoma state trooper (who was tricked by Lord Tigress, posing as Maya). Lord Tigress and Dracula in turn steal a helicopter to pursue Yo Gabba Gabba, who are picked up and given a ride by Scott and Edward, two students at Rocky and Bullwinkle's old university, Wossamotta U. Lord Tigress and Dracula arrive first and make a large donation to the university in Brobee and Muno's name. In return, the head university gives Brobee and Muno an honorary "Gabba's Degree" and Brobee and Muno addresses the student body, whilst Dracula attempts to kill them with the CDI from a water tower. Plex recovers his practice to fly and saves the oblivious Brobee and Muno. Scott and Edward lend the five their RV, which Brobee drives to Chicago. Lord Tigress and Dracula once again attempt to kill the five but instead accidentally destroy their helicopter.