- Chris Pratt as Robert Horse
- Tom Holland as Captain Tiger
- Julia Louis-Dreyfus as Little Anna
- Octavia Spencer as Betty Smith
- Zac Efron as Emeritus "Ellery" Chipmunk
- Amanda Seyfried as Dorothy Turtle
- Gina Rodriguez as Sally the Magical Mouse
- Will Forte as Peter Jaguar
- Tracy Morgan as Bucky Beaver
- Ken Jeong as Jacky the Penguin
- Kiersey Clemons as Maggie the Little Ghost
- Henry Winkler as Spirit Light
- Jim Meskimen as TV Announcer
- Dwayne Johnson as Owl Oswald
- Boris Johnson as himself (uncredited)
- Donald Trump as himself (uncredited)
- Matt Berry as Scripp Mouse
- Keanu Reeves as Boss Mouse
- Reggie Watts as Ugh
- Danny Trejo as Boots
- John Malkovich as Hero (uncredited)
- Shuya Chang as Hero Girl (uncredited)
- Marko Zaror as Evil Guy (uncredited)
- Scott McCord
- Drew Nelson
- Deven Mack
- Emilie-Claire Barlow
- Katie Crown
- Cory Doran
- Sarah Gadon
- Christian Potenza
- Kristin Fairlie
- Lilly Bartlam
- Bahia Watson
- Darren Frost
- Wyatt White
- Evany Rosen
- This is the tenth Shires Animation Studios film to feature the full 2011 Shires opening logo as a closing logo.
- This is the twelfth Shires Animation Studios feature to have the "Created and Produced at Shires Animation Studios, www.shires.com" credit at the end.
- The eigthth film with the Shires logo under the movie title.
- This is the fifth Shires animated feature to show only the studio logos in the beginning, and the title card and credits only appear at the end.
- The second Shires animated theatrical feature to receive a R rating.
- This is considered the return of Dorothy Turtle from Sidekick Express.
- The film was rated R for Motion Picture Association of America.
- The film was released on August 12, 2024.
- A video game based on the film, titled Light Bugs Movie Land, and published by Shires Interactive Studios, will be released on July 24, 2024 for Nintendo 3DS, Nintendo Switch, PlayStation 4 and Xbox One.
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